Whole cell kinetics of ureolysis by S porosarcina pasteurii

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Whole cell kinetics of ureolysis by Sporosarcina pasteurii.

AIMS Ureolysis drives microbially induced calcium carbonate precipitation (MICP). MICP models typically employ simplified urea hydrolysis kinetics that do not account for cell density, pH effect or product inhibition. Here, ureolysis rate studies with whole cells of Sporosarcina pasteurii aimed to determine the relationship between ureolysis rate and concentrations of (i) urea, (ii) cells, (iii...

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Urease is an important virulence factor from Helicobacter pylori that enables bacterial colonization of human gastric mucosa. Specific inhibition of urease activity can be regarded as a promising adjuvant strategy for eradication of this pathogen. A group of organophosphorus inhibitors of urease, namely, aminophosphinic acid and aminophosphonic acid derivatives, were evaluated in vitro against ...

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Biocalcification of Sand through Ureolysis

Biological processes may provide great and previously unexplored opportunities for cost-effective, in situ improvement of the engineering properties of soil. A laboratory study was conducted to evaluate the changes in geomechanical properties of sand attributable to the formation of calcium precipitates induced through ureolysis catalyzed by Sporosarcina pasteurii (S. pasteurii). Specifically, ...

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Sporosarcina pasteurii can form nanoscale crystals on cell surface

Using a semi-solid 0.5% agar column, we study the phenomenon of microbially induced mineral (calcium carbonate) by the bacteria Sporosarcina pasteurii. Our platform allows for in-situ visualization of the phenomena, and we found clear evidence of bacterial cell surface facilitating formation of nanoscale crystals. Moreover, in the bulk agar we found the presence of microspheres, which seem to a...

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ژورنال

عنوان ژورنال: Journal of Applied Microbiology

سال: 2015

ISSN: 1364-5072,1365-2672

DOI: 10.1111/jam.12804